06 Nov A Simple and Sensitive LC-MS/MS Method For Quantification of 7α-hydroxy-4-cholesten-3-one (C4) in Human Serum
I would like to thank Aiping Zhu, Tian-Sheng Lu, and Shuyu Hou from Medpace Bioanalytical Laboratories, for sharing their poster from ASMS 2024. “A Simple and Sensitive LC-MS/MS Method for Quantification of 7α-hydroxy-4-cholesten-3-one (C4) in Human Serum.” I found the application particularly interesting as it shows how Pentafluorophenyl (PFP) HPLC Columns can be used. The following is directly highlighted from the poster.
Medspace developed a fast and reliable LC-MS/MS method to measure 7α-Hydroxy-4-cholesten-3-one (C4), a key marker for diagnosing bile acid malabsorption (BAM)—often linked to chronic diarhhea and irritable bowel syndrome. This validated method is highly sensitive, accurate, and efficient, allowing the analysis of thousands of clinical samples. It meets Good Clinical Practice (GCP) standards and offers stability and precision, making it a valuable tool in clinical research.
