21 Jun Cautions on Connecting HPLC/UHPLC Columns and Fittings Features of the MarvelX and MarvelXACT Tubing
The volume of tubing and dead volume generated at the connecting points greatly affects the column performance. This is important as this 1/16″ steel tubing connects the components of the HPLC / UHPLC systems (pumps, autosamplers, injectors, columns and detectors). We will discuss several important issues to consider when connecting the column end fittings and how to avoid these issues. We will also introduce tubing that can be connected regardless of the end fitting standard.
The following video and technical note highlight this in more detail.
It is important to make tubing connections that meet the column
end fitting specifications in order to achieve the best column performance. It is also
important to fully understand the differences between the end-fitting standards of different
companies, which are not always easy to see. Ferrule-free tubing, such as MarvelX, does
not depend on column end fitting standards, and MarvelXACT is a convenient tubing
system that can be tightened to the same torque by anyone. Since dead volume
significantly affects the column performance, especially on 2.1 mm ID columns, we recommend using MarvelX or MarvelXACT with an ID of 0.075 mm, which can be
used under high pressure, enabling the columns to perform at their full potential.