Comparison between normal and semi-micro HPLC

Effect of inner diameter of tubing

The above theoretical plate was compared changing the inner
diameter of tubing between a column and a flow cell of the detector. A tubing with a large inner diameter has a large dead volume, so that it makes the peak width be wide. As a result, theoretical plate decreases. I recommend to use the tubing with 0.1 mm or less than 0.1 mm inner diameter for core shell columns

Effect of response time of detector

The response time of a detector is important. Regarding uracil, the real peak width is less than 0.8 sec. When the peak width is less than 1 sec, 0.03 sec of response time is needed. Furthermore, the sampling rate of an integrator should be set to be 0.1 sec.