17 Aug Metabolic Profiling of Cancer Biomarkers from Methanol Extracts from Pathology Specimens using LC-MS/MS

I want to thank Alexandra Izydorczak, Troy Wood and  Wilfrido Mojica for sharing their poster Metabolic Profiling of Cancer Biomarkers from Methanol Extracts from Pathology Specimens using LC-MS/MS from ASMS 2023. Although we are not related to this poster, other than receiving it, I found the chromatography and method interesting. 

The following is directly highlighted from the poster:

Introducing a Breakthrough in Cancer Biomarker Research.
• Pathologists have long studied biomarkers using traditional formalin-fixed, paraffin-embedded (FFPE) samples, but a new technique using methanol extraction and preservation method for fixation (mPREF) promises revolutionary advancements.

 Highlights:
• Innovative Preservation: Huan et al. discovered a non-destructive method preserving tissue structure while extracting metabolites from various tissue types.
Potential Biomarker Identification: The mPREF technique shows promise in identifying metabolic cancer biomarkers.
Prostate Tissue Focus: The study showcases successful application on enlarged prostate tissue.
Optimized LC-MS Method: The research achieved reproducible metabolite separation with LC-MS/MS.

Methods & Results:
• The study involved extracting and concentrating methanol from prostate tissue samples.
The optimized method demonstrated consistent chromatogram profiles with similar peak shapes and retention times.

Conclusions & Future Work
• Figures 2 and 3 reveal the optimal method for metabolite identification.
Further optimization of the SPE method for elution solvent is recommended and will continue working on this.
The next steps include applying the technique to bladder cancer samples for potential biomarker identification.
 This research holds the potential for advancing cancer biomarker determination.

Please see the poster for more details here.