iSPE-HILIC cartridges are designed for the purification of polar and hydrophilic compounds using Hydrophilic Interaction Liquid Chromatography (HILIC). The packed stationary-phases are charge-modulated hydroxyethyl amide silicas which are covalently bonded with neutral, positively-charged, and negatively-charged hydrophilic functional groups. Therefore, the separation mechanism with iHILIC columns is based on a combination of hydrophilic interaction, hydrogen bonding, and weak electrostatic interactions. They have unique separation selectivity and high separation efficiency. iSPE-HILIC can be used for the purification of glycopeptides, glycans, PSP toxins, and many other polar compounds.