This shows two cases of isomers separation using C30, PFP and C18 stationary phases. Regarding the separation of m-and p-xylene, only C30 stationary phase can separate them. C18 and PFP stationary phases cannot separate them. On the other hand, regarding m- and p-cresol, C18 stationary phase can not separate them. PFP and C30 phases can separate them. However, C30 stationary phase needed THF in the mobile phase and a long column and took a long time to separate them.
It is considered that PFP tends to show better separation than C30 when the difference in electronegativity of two additional groups on the benzene ring is large.
This shows the separation of o, m, and p-toluidine using PFP, Phenyl and C18 stationary phases. When acetonitrile and 10 mM ammonium acetate pH 6.8 10 to 90 were used as a mobile phase, any stationary phase can separate them, although separation is not enough. When methanol and 10 mM ammonium acetate pH 6.8 10 to 90 were used as a mobile phase, the PFP column showed a complete separation although phenyl and C18 columns could not separate m- and p- toluidine.
This slide shows the separation of vitamin E isomers at different temperatures using the SunShell C30 column. As a reference, separation by the C18 column is shown at the bottom. The C18 column could not separate beta and gamma-tocopherol which were isomers. But the C30 column could separate them. Regarding temperature, the lower the temperature, the higher the separation factor.
This slide shows the separation of vitamin K1 using SunShell C30, company T Accucore C30 and SunShell C18 columns. The C18 column could not separate vitamin K1 trans and cis as same as Vitamin E, beta and gamma-tocopherol.
However, the C30 column could separate them.
This slide is the last one for the separation of isomers. o-, m-, p-terphenyl were separated. The C18 column could separate m- and p-terphenyl. However C30 column showed a much greater resolution of m- and p-terphenyl than the C18 column.