SunShell 2-EP 2.6 μm core-shell column shows only one-third of back pressure to compare with the 1.7 μm fully porous column although both show almost the same efficiency. By such low back pressure, the difference in density of supercritical fluid between an inlet and an outlet of the column is reduced. Consequently, the 2.6 μm core-shell column performs a superior separation for SFC.
Characteristics of SunShell 2-EP
Core-shell silica
Particle size
Pore diameter
Specific surface area
Carbon content
Bonded phase
End-capping
Maximum operating pressure
Available pH range
SunShell 2-EP
2.6 μm
9 nm
150 m2/g
2.5%
2-Ethylpyridine
no
60MPa or 8,570 psi
2-7.5
Comparison between SunShell 2-EP and 1.7 μm fully porous 2-EP
Figure 1: Chromatogram of the separation for he 17- component mix using the Sun Shell 2-EP 150 x 3.0 mm column. A methanol gradient of < 2 minutes was used on the Agilent 1260 Infinity SFC system. SFC conditions: flow rate: 4.0mL/min; outlet pressure 160 bar; column temperature 55⁰C. Gradient program: 5.0- 7.5% in 0.20 min, then 7.5-20% in 1.3 min and held at 20% for 0.2 min.
Figure 2: Chromatogram of the separation for the 17-component mix using Acquity UPC2 Viridis 2-EP 100 x 3.0 mm column. 16 of the 17 components were resolved. A methanol gradient of < 2 minutes was used on the Agilent 1260 Infinity SFC system. SFC conditions: flow rate 3.5 mL/min; outlet pressure 160 bar; and column temperature 70⁰C. Gradient program: 5.0-12.5% in 1.0 min, 12.5% for 0.25 min, then 12.5-20% in 0.75 min.
