L- column Micro is a nano/microcolumn with 0.075-0.3 mm I.D. It is a high-performance column that was manufactured by combining low-adsorption packing materials, including L-column2 ODS, an originally developed packing technique, and a column structure with a small dead volume. L-column Micro shows an advantage at all concentration levels but excels at the lower levels. With superior end-capping and very high theoretical plate numbers per column, L-column Micro permits the identification of many proteins and is the optimum choice for performance analysis. L-column can separate many peptides because of the low-adsorption packing materials. This is effective for the identification of proteins. The column has a high theoretical plate number and high durability because it is packed homogeneously by a patented technique.

LC column L-column series


L- Column

L-column ODS was introduced in 1990 as a column that eliminates secondary interactions due to residual silanol groups by the high-temperature vapor-phase end-capping method. Outstanding performance compared to commercially available ODS columns at that time was positioned as a pioneer column. It is a column that can be used with confidence due to a stable supply for many years.

Physical Property Information L-column C18 ODS L-column C8
Base material Porous high-purity spherical silica Particle size 3μm, 5μm Particle size 5μm
Pore diameter 12nm Modifying group Octadecyl group Modifying group Octyl group
Specific surface area 340 m 2 / g End capping High temperature gas phase end capping End capping  
  USP category L1 USP category L1
Working pH range pH 2 to pH 9 Working pH range pH 2 to pH 9

Certificated L-column ODS-V (particle size 5 μm only)
L-column ODS-V has a “Certificate of analysis” attached to the L-column ODS.
“Certificate of analysis” proves that the physical property value and separation behavior of the lot is within the standard. Supports analytical method validation.


L-column2

L-column2 is a high-performance column that minimizes the effects of residual silanol groups. From acidic substances to basic substances to coordination compounds, sharp peaks can be obtained without tailing with a simple method. We have a rich lineup of phenyl columns, microcolumns and nanocolumns with an inner diameter of less than 1 mm. A metal-free column (glass lining) is also available.

Physical Property Information L-column2 C18 ODS L-column2 C8 L-column2 C6-Phenyl
Base Material Porous high-purity spherical silica Particle size 2μm, 3μm, 5μm Particle size 2μm, 3μm, 5μm Particle size 2μm, 3μm, 5μm
Pore diameter 12nm Modifying group Octadecyl group Modifying group Octyl group Modifying group Phenylhexyl group
Specific surface area 340m 2/g End capping Advanced end capping End capping Advanced end capping End capping Advanced end capping
  USP category L1 USP category L7 USP category L11
Working pH range pH1 to pH9 Working pH range pH2 to pH7.5 Working pH range pH2 to pH7.5

L-column3

L-column3 has achieved a dramatic improvement in the chemical stability (acid resistance, alkali resistance) of the filler with the newly developed PCS silica and durable advanced end-capping. It is an all-around column that allows you to freely design any method.

Physical Property Information L- column3 C18 L- column3 C8
Base material PCS silica (porous high-purity spherical silica) Particle size 2μm, 3μm, 5μm Particle size 3μm, 5μm
Pore Diameter 12 nm Modifying group Octadecyl group Modifying group Octyl group
Specific surface area 340m 2/g End capping Durable advanced end-capping End-capping Durable advanced end-capping
  USP category L7 USP category L7
Working pH range pH1 to pH12 Working pH range pH1 to pH12

PCS Silica (Perfect Chemical Stable Silica)
A highly chemically durable base material that is resistant to erosion even in alkaline solutions.

Durable advanced end-capping
Advanced end-capping ( adopted for L-column 2 ) reduces silanol groups to the utmost limit and coats the silica gel surface with high density to prevent secondary interaction with silanol groups and metal impurities. It is a technology that eliminates the limit. The evolution of this is the “durable advanced end-capping”, which has further improved low adsorption and durability due to inactivation.