L- column Micro is a nano/microcolumn with 0.075-0.3 mm I.D. It is a high-performance column that was manufactured by combining low-adsorption packing materials, including L-column2 ODS, an originally developed packing technique, and a column structure with a small dead volume. L-column Micro shows an advantage at all concentration levels but excels at the lower levels. With superior end-capping and very high theoretical plate numbers per column, L-column Micro permits the identification of many proteins and is the optimum choice for performance analysis. L-column can separate many peptides because of the low-adsorption packing materials. This is effective for the identification of proteins. The column has a high theoretical plate number and high durability because it is packed homogeneously by a patented technique.
L-column ODS was introduced in 1990 as a column that eliminates secondary interactions due to residual silanol groups by the high-temperature vapor-phase end-capping method. Outstanding performance compared to commercially available ODS columns at that time was positioned as a pioneer column. It is a column that can be used with confidence due to a stable supply for many years.
|Physical Property Information||L-column C18 ODS||L-column C8|
|Base material||Porous high-purity spherical silica||Particle size||3μm, 5μm||Particle size||5μm|
|Pore diameter||12nm||Modifying group||Octadecyl group||Modifying group||Octyl group|
|Specific surface area||340 m 2 / g||End capping||High temperature gas phase end capping||End capping|
|USP category||L1||USP category||L1|
|Working pH range||pH 2 to pH 9||Working pH range||pH 2 to pH 9|
Certificated L-column ODS-V (particle size 5 μm only)
L-column ODS-V has a “Certificate of analysis” attached to the L-column ODS.
“Certificate of analysis” proves that the physical property value and separation behavior of the lot is within the standard. Supports analytical method validation.
L-column2 is a high-performance column that minimizes the effects of residual silanol groups. From acidic substances to basic substances to coordination compounds, sharp peaks can be obtained without tailing with a simple method. We have a rich lineup of phenyl columns, microcolumns and nanocolumns with an inner diameter of less than 1 mm. A metal-free column (glass lining) is also available.
|Physical Property Information||L-column2 C18 ODS||L-column2 C8||L-column2 C6-Phenyl|
|Base Material||Porous high-purity spherical silica||Particle size||2μm, 3μm, 5μm||Particle size||2μm, 3μm, 5μm||Particle size||2μm, 3μm, 5μm|
|Pore diameter||12nm||Modifying group||Octadecyl group||Modifying group||Octyl group||Modifying group||Phenylhexyl group|
|Specific surface area||340m 2/g||End capping||Advanced end capping||End capping||Advanced end capping||End capping||Advanced end capping|
|USP category||L1||USP category||L7||USP category||L11|
|Working pH range||pH1 to pH9||Working pH range||pH2 to pH7.5||Working pH range||pH2 to pH7.5|
L-column3 has achieved a dramatic improvement in the chemical stability (acid resistance, alkali resistance) of the filler with the newly developed PCS silica and durable advanced end-capping. It is an all-around column that allows you to freely design any method.
|Physical Property Information||L- column3 C18||L- column3 C8|
|Base material||PCS silica (porous high-purity spherical silica)||Particle size||2μm, 3μm, 5μm||Particle size||3μm, 5μm|
|Pore Diameter||12 nm||Modifying group||Octadecyl group||Modifying group||Octyl group|
|Specific surface area||340m 2/g||End capping||Durable advanced end-capping||End-capping||Durable advanced end-capping|
|USP category||L7||USP category||L7|
|Working pH range||pH1 to pH12||Working pH range||pH1 to pH12|
PCS Silica (Perfect Chemical Stable Silica)
A highly chemically durable base material that is resistant to erosion even in alkaline solutions.
Durable advanced end-capping
Advanced end-capping ( adopted for L-column 2 ) reduces silanol groups to the utmost limit and coats the silica gel surface with high density to prevent secondary interaction with silanol groups and metal impurities. It is a technology that eliminates the limit. The evolution of this is the “durable advanced end-capping”, which has further improved low adsorption and durability due to inactivation.