L- column Micro is a nano/micro column with 0.075-0.3 mm I.D. It is a high-performance column that was manufactured by combining low-adsorption packing materials, including L-column2 ODS, an originally developed packing technique, and a column structure with a small dead volume. L-column Micro shows an advantage at all concentration levels but excels at the lower levels. With superior end-capping and very high theoretical plate numbers per column, L-column Micro permits the identification of many proteins and is the optimum choice for performance analysis. L-column can separate many peptides because of the low-adsorption packing materials. This is effective for the identification of proteins. The column has a high theoretical plate number and high durability because it is packed homogeneously by a patented technique.

LC column L-column series

L- Column

L-column ODS was introduced in 1990 as a column that eliminates secondary interactions due to residual silanol groups by the high-temperature vapor-phase end-capping method. Outstanding performance compared to commercially available ODS columns at that time was positioned as a pioneer column. It is a column that can be used with confidence due to stable supply for many years.

Physical Property InformationL-column C18 ODSL-column C8 
Base materialPorous high-purity spherical silicaParticle size3 μm, 5 μmParticle size5 μm
Pore diameter12nmModifying groupOctadecyl groupModifying groupOctyl group
Specific surface area 340 m 2 / gEnd cappingHigh temperature gas phase end cappingEnd capping
USP categoryUSP category
Working pH rangepH 2 to pH 9Working pH range

Certificated L-column ODS-V (particle size 5 μm only)
L-column ODS-V has a “Certificate of analysis” attached to the L-column ODS.
“Certificate of analysis” proves that the physical property value and separation behavior of the lot is within the standard. Supports analytical method validation.


L-column2 is a high-performance column that minimizes the effects of residual silanol groups. From acidic substances to basic substances to coordination compounds, sharp peaks can be obtained without tailing with a simple method. We have a rich lineup of phenyl columns, micro columns and nano columns with an inner diameter of less than 1 mm. A metal-free column (glass lining) is also available.

Physical Property InformationL-column2 C18 ODSL-column2 C8L-column2 C6-Phenyl 
Base MaterialPorous high-purity spherical silicaParticle size2 μm, 3 μm, 5 μmParticle size3 μm, 5 μmParticle size3 μm, 5 μm
Pore diameter12nmModifying groupOctadecyl groupModifying groupOctyl groupModifying groupPhenylhexyl group
Specific surface area340 m 2 / gEnd cappingAdvanced end cappingEnd cappingAdvanced end cappingEnd cappingAdvanced end capping
USP categoryL1USP categoryL 7USP categoryL11
Working pH rangepH1 to pH9Working pH rangepH2 to pH7.5Working pH rangepH2 to pH7.5


L-column3 has achieved a dramatic improvement in the chemical stability (acid resistance, alkali resistance) of the filler with the newly developed PCS silica and durable advanced end capping. It is an all-round column that allows you to freely design any method.

Physical Property InformationL- column3 C18L- column3 C8
Base materialPCS silica (porous high-purity spherical silica)Particle size2 μm, 3 μm, 5 μmParticle size3 μm, 5 μm
Pore Diameter12 nmModifying groupOctadecyl groupModifying groupOctyl group
Specific surface area340 m 2 / gEnd cappingDurable advanced end cappingEnd cappingDurable advanced end capping
USP categoryL7USP categoryL7
Working pH rangepH1 to pH12Working pH rangepH1 to pH12

PCS Silica (Perfect Chemical Stable Silica)
A highly chemically durable base material that is resistant to erosion even in alkaline solutions.

Durable advanced end-capping
Advanced end-capping ( adopted for L-column 2 ) reduces silanol groups to the utmost limit and coats the silica gel surface with high density to prevent secondary interaction with silanol groups and metal impurities. It is a technology that eliminates the limit. The evolution of this is the “durable advanced end-capping”, which has further improved low adsorption and durability due to inactivation.