2.6 μm core shell column shows only one-third of back pressure to compare with 1.7 μm fully porous column although both show almost the same efficiency. By such low back pressure, a difference of density of supercritical fluid between an inlet and an outlet of the column is reduced. Consequently, 2.6 μm core shell column performs a superior separation for SFC.
Characteristics of SunShell 2-EP
Core shell silica
Particle size
Pore diameter
Specific surface area
Carbon content
Bonded phase
End-capping
Maximum operating pressure
Available pH range
SunShell 2-EP
2.6 μm
9 nm
150 m2/g
2.5%
2-Ethylpyridine
no
60MPa or 8,570 psi
2-7.5
Comparison between SunShell 2-EP and 1.7 μm fully porous 2-EP
Figure 1: Chromatogram of the separation for he 17- component mix using the Sun Shell 2-EP 150 x 3.0 mm column. A methanol gradient of < 2 minutes was used on the Agilent 1260 Infinity SFC system. SFC conditions: flow rate: 4.0mL/min; outlet pressure 160 bar; column temperature 55⁰C. Gradient program: 5.0- 7.5% in 0.20 min, then 7.5-20% in 1.3 min and held at 20% for 0.2 min.
Figure 2: Chromatogram of the separation for the 17-component mix using Acquity UPC2 Viridis 2-EP 100 x 3.0 mm column. 16 of the 17 components were resolved. A methanol gradient of < 2 minutes was used on the Agilent 1260 Infinity SFC system. SFC conditions: flow rate 3.5 mL/min; outlet pressure 160 bar; and column temperature 70⁰C. Gradient program: 5.0-12.5% in 1.0 min, 12.5% for 0.25 min, then 12.5-20% in 0.75 min.
